DNA Replication and DNA Storage Practice Test

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Which process fills gaps left after RNA primers are removed during replication?

Ligases alone seal the gaps.

Topoisomerases fill in with nucleotides.

DNA polymerases fill in the gaps with DNA and DNA ligase seals.

When RNA primers are removed during replication, the gaps are filled by DNA polymerase synthesizing DNA in place of the RNA, and then DNA ligase seals the remaining nicks to create a continuous strand. This means the combination of filling with DNA and sealing the backbone is the correct process. Ligase alone can seal but cannot add nucleotides; topoisomerase relieves torsional strain, not fill nucleotides; primers aren’t added again after primer removal.

Primers are added again.

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